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CURRICULUM VITAE
Ping LI, Ph.D.
212 Biology Unit I, Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
Email: pli@bio.fsu.edu
EDUCATION & WORK EXPERINCE
Nov.2009- Postdoctor
Florida State University, Department of Biological Science, Tallahassee, FL.
Advisor: Dr. Hong-Guo Yu.
2009 Doctorˇs degree in Microbiology
Zhejiang University, College of Life science, Hangzhou, Zhejiang.
Advisor: Dr. Xue-Chang Wu, and Dr. Hang Min.
2003 Bachelorˇs degree in Biological sciences
Shaoxing University, College of Life science, Shaoxing, Zhejiang.
PUBLICATIONS
[1] Ping Li, Yize Shao, Hui Jin, Hong-Guo Yu∗. Ndj1, a telomere-associated protein, regulates centrosome separation in budding yeast meiosis, The Journal of Cell Biology, accepted. 5-year IF: 10.398.
[2] Ping Li, Hui Jin, Hong-Guo Yu∗. Condensin suppresses recombination and regulates double-strand break processing at the repetitive rDNA array to ensure proper chromosome segregation during meiosis in budding yeast, Molecular biology of the cell, 2014, 25 (19): 2934-47. 5-year IF: 5.154.
[3] Fengzhi Jin1, Hong Liu1 , Ping Li, Hong-Guo Yu, Yanchang Wang∗. Loss of function of the Cik1/Kar3 motor complex results in chromosomes with syntelic attachment that are sensed by the tension checkpoint, PLoS Genetics, 2012, 8(2).
[4] Ping Li, Hui Jin, Margaret L Hoang, Hong-Guo Yu∗. Tracking chromosome dynamics in live yeast cells: coordinated movement of rDNA homologs and anaphase disassembly of the nucleolus during meiosis, Chromosome research, 2011; 19(8):1013-26.
[5] DaoQiong Zheng, XueChang Wu∗, XiangLin Tao, PinMei Wang, Ping Li, XiaoQin Chi, YuDong Li, QingFeng Yan, YuHua Zhao. Screening and construction of Saccharomyces cerevisiae strains with improved multi-tolerance and bioethanol fermentation performance, Bioresource Technology, 2011, 102(3): 3020-3027.
[6] DaoQiong Zheng, XueChang Wu∗, PinMei Wang, XiaoQin Chi, XiangLin Tao, Ping Li, XinHang Jiang, YuHua Zhao. Drug resistance marker-aided genome shuffling to improve acetic acid tolerance in Saccharomyces cerevisiae, J Ind Microbiol Biotechnol, 2011; 38(3):415-22.
[7] XueChang Wu∗,1, Ping Li1 (co-first author), ChaoDong Qian, Ou Li,YinYan Zhou. Trimeric coiled coil domain from human Pulmonary surfatcant-associated protein D enhance biological activity and stability of TRAIL, Molecular Immunology, 2009, 46: 2381-2388.
[8] Ping Li, JianYing Zhou, YinYan Zhou, ChaoDong Qian, Ou Li, Hang Min∗, XueChang Wu∗. The Coiled-coil Neck Domain of Human Pulmonary Surfactant Protein D Drives Trimerization and Stabilization of Thioredoxin, a Heterologous Non-collagenous Protein. Protein Pept Lett, 2009,16(3):306-311.
[9] Yinyan Zhou, Ping Li, XueChang Wu∗. Research advancement of TRAIL in tumor apoptosis. Bulletin of Science and Technology. 2009, 25(3):288-293 (in Chinese).
[10] GenFu Wu∗, Ping Li, XueChang Wu. Regulation of Escherichia coli IscS desulfurase activity by ferrous iron and cysteine. Biochemical and Biophysical Research Communications. 2008, 374:399- 404.
[11] XueChang Wu∗, WeiFeng Chen, ChaoDong Qian, Ou Li, Ping Li, YanPing Wen. Isolation and Identification of Newly Isolated Antagonistic Streptomyces sp. Strain AP19-2 Producing Chromomycins. The Journal of Microbiology, 2007, 45(6): 499-504.
[12] Ping Li, XueChang Wu∗. Molecular Mechanisms of Resistance to TRAIL-induced Apoptosis in Tumor. Chinese Journal of Cell Biology, 2006, 28:153-159.
[13] Junxia Yin∗, Wenying Shen, Ping Li. Study on the influence of water temperature on the intestinal microfiora of Penaeus vannamei. Marine Sciences, 2004, 28(5) :33-36.
TECHNICAL SKILLS
Microbiology
劔 Microorganism cultivation: Multiple aerobes, anaerobes and facultative aerobes;
劔 Strain screening and identification: 16S rDNA sequence analysis, evolutionary tree construction;
劔 Fermentation process optimization of industrial strains: Shaking flask cultivation and bioreactor fermentation, Response surface methodology for optimization.
Molecular biology
劔 Construction of genetic engineering strains: construct engineering strains using E.coli, Pichia pastopris, Shingomonas.sp and S.cerevisiae as hosts; skillful in several PCR techniques; comprehend screening techniques such as chemical mutation, ultraviolet mutation and gene rearrangement.
劔 Expression and purification of recombinant proteins: positive or negative ion exchange chromatography, affinity chromatography, hydrophobic chromatography, SDS-PAGE, Western blotting, etc.
劔 Characterization of proteins: circular dichroism spectroscopy (CD), HPLC, Chemical cross-linking, SEC-HPLC, ESI-MS, atomic absorption spectrometry, etc.
Cell biology
劔 Cultivation of tumor cells; MTT assay, ELISA (Enzyme-Linked ImmunoSorbent Assay), Sandwich ELISA
劔 Animal experiments: Tail intravenous injection and eyeball blood sampling etc.
劔 Immunofluorescence
Microscopy
劔 Time-lapse 3-D fluorescence microscopy
劔 Fluorescence microscopy, and other
Bioinformatics and software
劔 Proficiency in several professional data analysis software such as Primer Premier 5.0, Bandscan 4.0, RNAstructure 4.3, DNAman 5.2.2, OMIGA 2.0, ANTHEWIN V5.0, Mega 4.0, Clustal W, Design Expert 7.0;
劔 Proficiency in several image and file editing software such as Endnote x2, Origin 7.0, Smartdraw 7.
RESEARCH EXPERIENCE
2009- Condensin suppresses rDNA recombination and is required for rDNA separation in meiosis I
Ndj1regulates SPB separation in yeast meiosis I
2004- 2009 Tumor targeting therapy of ST
Cloning, expression, antitumor activity and mechanisms analysis of ST
2008- 2009 Construction of high-yield gellan gum engineering strains.
Collected microorganisms for screening cellulose-decomposing strains or high-yield Saccharomyces cerevisiae strains in Hainan Province
Scientific research on the diversity of edaphon in Tianmu Mountains
2003-2004 Studied heat shock protein and thermotolerance mechanism of Saccharomyces cerevisiae.
2002- 2003 Influence of water temperature on the intestinal microfiora of Penaeus vannamei.
AWARDS
2003- 2009 Excellent Student Cadre of Zhejiang University;
Outstanding graduate student of Zhejiang University;
The Second Prize of graduate student scholarship of Zhejiang University.
1999- 2003 First prize scholarship (1/40, twice) or second prize scholarship (3/40, twice) every year;
All-round Excellent Student (1/40, twice);
Outstanding Student of 2003 Graduating Class of Shaoxing University;
Excellent League Member (1/40, twice).
Champion in the college Teaching-skilling match;
Take part in volleyball and basketball game, our team won the champion;
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