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Professor Evert Johan Breukink(Eefjan) obtained a Ph.D. in Biochemistry from the University of Utrecht in the Netherlands in 1994. He currently serves as the Director of the Department of Membrane Biochemistry and Biophysics at the University of Utrecht in the Netherlands, and is a top expert in membrane biology and antimicrobial peptides worldwide. Eefjan has been engaged in the study of the structure and mechanism of action of antimicrobial peptides for a long time, and has discovered for the first time in the world the antibacterial mechanism of nisin, the only lactobacillus bacteriocin allowed for use in food.
Education:
1983-1989 Chemistry with a major in Biochemistry at the University of Utrecht.
Professional Experience:
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2014-present Honorary Professor of the Zhejiang Provincial Key Laboratory of Food Microbiotechnology Research of China.
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2010-2014: Co-founder, CTO and ad interim CEO (October 2013 - April 2014) of QVQ
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2003-present: Associate professor (UHD) at the department Membrane Biochemistry & Biophysics.
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2000-2003: Postdoctoral position at the department Biochemistry of membranes, University of Utrecht.
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1999: Postdoctoral position at the Biomembrane Structure Unit of the Department of Biochemistry (Prof. Dr. A. Watts), University of Oxford.
List of publications:
1. Slootweg JC, van Schaik TB, Quarles van Ufford HL, Breukink E, Liskamp RM, Rijkers DT. (2013). Improving the biological activity of the antimicrobial peptide anoplin by membrane anchoring through a lipophilic amino acid derivative. Bioorg Med Chem Lett. 2013 23:3749-3752.
2. Imran M, Revol-Junelles AM, de Bruin M, Paris C, Breukink E, Desobry S. (2013) Fluorescent labeling of nisin Z and assessment of anti-listerial action. J Microbiol Methods. Nov;95(2):107-13. doi: 10.1016/j.mimet.2013.07.009.
3. Varney KM, Bonvin AM, Pazgier M, Malin J, Yu W, Ateh E, Oashi T, Lu W, Huang J, Diepeveen-de Buin M, Bryant J, Breukink E, Mackerell AD Jr, de Leeuw EP. (2013) Turning Defense into Offense: Defensin Mimetics as Novel Antibiotics Targeting Lipid II. PLoS Pathog. Nov;9(11):e1003732. doi: 10.1371/journal.ppat.1003732.
4. Zapun A, Philippe J, Abrahams KA, Signor L, Roper DI, Breukink E, Vernet T. In vitro Reconstitution of Peptidoglycan Assembly from the Gram-Positive Pathogen Streptococcus pneumoniae. (2013) ACS Chem Biol. Sep 30. [Epub ahead of print]
5. Rijkers DT, Slootweg J, van der Wal S, Quarles van Ufford LC, Breukink E, Liskamp RM. (2013) Synthesis, Antimicrobial Activity, and Membrane Permeabilizing Properties of C-Terminally Modified Nisin Conjugates Accessed by CuAAC. Bioconjug Chem. Nov 25. [Epub ahead of print]
6. Mohammadi T, Sijbrandi R, Lutters M, Verheul J, Martin NI, den Blaauwen T, de Kruijff B, Breukink E. (2014) Specificity of the transport of lipid II by FtsW in Escherichia coli. J Biol Chem. 289:14707-14718.
7. van Vught R, Pieters RJ, Breukink E. (2014) Site-specific functionalization of proteins and their applications to therapeutic antibodies. Comput. Struct. Biotechnol. J. e201402001.
8. Egan AJ, Jean NL, Koumoutsi A, Bougault CM, Biboy J, Sassine J, Solovyova AS, Breukink E, Typas A, Vollmer W, Simorre JP. (2014) Outer-membrane lipoprotein LpoB spans the periplasm to stimulate the peptidoglycan synthase PBP1B. Proc Natl Acad Sci U S A. 111:8197-8202.
9. Mohammadi T, Breukink E. (2014) The chlamydial anomaly clarified? Chembiochem. 15:1391-1392.
10. Slootweg JC, Peters N, Quarles van Ufford HL, Breukink E, Liskamp RM, Rijkers DT. (2014) Semi-synthesis of biologically active nisin hybrids composed of the native lanthionine ABC-fragment and a cross-stapled synthetic DE-fragment. Bioorg Med Chem. 22: 5345–5353.
11. Bury D, Dahmane I, Derouaux A, Dumbre S, Herdewijn P, Matagne A, Breukink E, Mueller-Seitz E, Petz M, Terrak M. (2015) Positive cooperativity between acceptor and donor sites of the peptidoglycan glycosyltransferase. Biochem Pharmacol. 93:141-150.
12. Scheidelaar S, Koorengevel MC, Pardo JD, Meeldijk JD, Breukink E, Killian JA. (2015) Molecular model for the solubilization of membranes into nanodisks by styrene maleic Acid copolymers. Biophys J. 108:279-290.
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